Node ReferenceInput / Output

Input / Output Nodes

I/O nodes bring data into a workflow and take results out. They are the entry and exit points of every graph.

Input nodes

FASTQ Input

Loads single- or paired-end sequencing reads.

ParameterTypeDescription
r1FileRead 1 (.fastq / .fastq.gz)
r2FileRead 2 (optional, for paired-end)
sampleStringSample name used in read groups

Outputs: reads: FASTQ

Reference Input (FASTA)

Provides a reference genome or transcriptome.

ParameterTypeDescription
fastaFileReference sequence (.fa / .fasta)
buildIndexBoolBuild aligner indices on load

Outputs: reference: FASTA

SRA / ENA Download

Fetches public reads by accession (e.g. SRR…, ERR…) directly into the run.

Outputs: reads: FASTQ

Generic File / Directory Input

Loads an arbitrary file or folder as a File / Directory for nodes that accept generic inputs.

Output nodes

VCF / BAM / FASTA Output

Marks an artifact as a workflow result. Output nodes make their upstream data downloadable from the run detail page and (in the cloud) persist it to versioned object storage.

ParameterTypeDescription
filenameStringName for the exported artifact
persistBoolKeep beyond the run’s TTL (cloud)

Report Output

Surfaces an HTML/PDF report (FastQC, MultiQC, QUAST) so it renders inline in the run view.

Tips

  • Connecting a node to an Output node is what makes its result downloadable — intermediate results are cached but not exported unless you ask for them.
  • Use SRA / ENA Download to build fully self-contained, shareable example workflows that need no local files.

See also